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. 2017 Nov 27;114(50):13242–13247. doi: 10.1073/pnas.1710433114

Fig. 6.

Fig. 6.

A single NAIP isoform is sufficient for inflammasome responses to flagellin, the T3SS inner rod protein, and the T3SS needle protein. (A and B) THP-1 cells were primed with Pam3CSK4 and treated with PA alone, LFn-FlaA310–475 alone, LFn-PrgJ alone, PA+LFn-FlaA310–475 (FlaTox), or PA+LFn-PrgJ (PrgJTox) for 5 h. IL-1α and IL-1β supernatant levels were measured by ELISA. Bar graphs display the mean ± SD of triplicate wells. Representative of three independent experiments. ***P < 0.001 by unpaired t test; ****P < 0.0001 by unpaired t test. (C) HEK293 cells were transfected with expression vectors encoding NLRC4, caspase-1, and IL-1β. Where indicated, cells were also transfected with vectors encoding NAIP* (+) or empty vector control (−). After 18 h, cells were treated with PA+LFn-PrgJ, PA+LFn-FlaA310–475, PA+LFn-YscF, or PA alone for 9 h. Immunoblot analysis was performed on cell lysates for mature and pro–IL-1β, NAIP*, NLRC4, caspase-1, and β-actin as a loading control. Representative of three independent experiments.