Figure 6.

Tigf2 is a target of miR-34a. (A) The results of bioinformatics. Wild-type or mutant Tigf2 3′UTR luciferase reporter constructs were cotransfected with miR-34a mimic or NC scramble-miR, and luciferase activities were assayed 24 h post-transfection. (B) Human umbilical vein endothelial cells were transfected with an miR-34a inhibitor or NC scramble-miR. Cell lysates were prepared and assessed by western blot analysis to detect the expression of TIGF2 and GAPDH (internal control).*P<0.05. Tigf2, transforming growth factor β-induced factor homeobox 2; miR, microRNA; UTR, untranslated region; NC, negative control.