Effects of CM-chitosan on the protein levels of iNOS, IL-10, JAK1, STAT3 and SOCS3 in LPS-induced rat chondrocytes. Rat chondrocytes were treated with LPS and subsequently increasing concentrations (50, 100 and 200 µg/ml) of CM-chitosan were added. (A) A western blot analysis was performed to detect the proteins present in the rat chondrocytes. These results were quantified and the relative protein expression levels of (B) iNOS, (C) IL-10, (D) JAK1, (E) STAT3 and (F) SOCS3 were presented. Data are presented as the mean ± standard deviation. #P<0.05 vs. the control group, *P<0.05 vs. the LPS group and **P<0.05 vs. the LPS+50 µg CM-chitosan group. CM, carboxymethyl; iNOS, inducible nitric oxide synthase; IL, interleukin; JAK, janus kinase; STAT, signal transducer and activator of transcription; SOCS suppressor of cytokine signaling; LPS, lipopolysaccharide.