Figure 4.

n-3 PUFA reduces primary cardiomyocyte apoptosis. (A) Hoechst staining (white arrows indicate apoptotic cells) and (B) flow cytometry were applied to determine apoptosis of primary cardiomyocytes isolated from H/M-Sod2^−/− mice. (C) Western blot analysis of the changed protein patterns after n-3 PUFA treatment in the hearts of H/M-Sod2^−/− mice. n=5; *P<0.05, **P<0.01 and ***P<0.001 vs. WT mice. n-3 PUFA, omega-3 polyunsaturated fatty acids; H/M-Sod2^−/−, heart/muscle-specific manganese-superoxide dismutase-deficient mice; WT, wild type; FITC, fluorescein isothiocyanate; PI, propidium iodide; JNK, c-Jun N-terminal kinases; NF, nuclear factor; c, cleaved; NOX4, nicotinamide adenine dinucleotide phosphate oxidase 4.