Figure 3.
Effects of GLP on DNA repair-associated proteins expression. HepG2 cells were treated with GLP (10 µM) for 72 h and then with radiation (6 Gy) for 30 min. Western blot analysis was performed to detect the protein expression of ATM and DNA-PK and their phosphorylation levels. GLP, Ganoderma lucidum polysaccharide; ATM, ataxia-telangiectasia mutated; DNA-PK, DNA dependent-protein kinase; IR, irradiation; p, phosphorylated; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.