Figure 4.

Akt is associated with GLP-regulated ATM activity in HepG2 cells. HepG2 cells were treated with GLP (10 µM) alone or combined with Akt inhibitor A-674563 for 72 h and then with radiation (6 Gy) for 30 min. Western blot analysis was performed to detect the protein expression of ATM and DNA-PK and their phosphorylation levels. Akt, protein kinase B; GLP, Ganoderma lucidum polysaccharide; ATM, ataxia-telangiectasia mutated; DNA-PK, DNA dependent-protein kinase; pDNA-PK, phosphorylated DNA-PK; pATM, phosphorylated-ATM; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.