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. 2017 Dec 21;12:62. doi: 10.1186/s13027-017-0173-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Detection of EBV in poorly differentiated invasive ductal carcinoma tissue section using EBER-ISH nuclear staining. Epstein-Barr virus-encoded RNA-1 (EBER1) in situ hybridization technique was used on those samples which were relatively more positive by PCR (n = 14) and signal for viral transcripts was observed in 35.71% (5/14). Figure 1a shows a case of poorly differentiated invasive ductal carcinoma exhibiting nuclear positivity indicated by dark staining within malignant cells (arrows). (X 40 objective). Figure. 1b is the hematoxyline and eosin staining of the image in Fig. 1a. (X 40 objective). Figure 1c is a positive control from a case of EBV infected nasopharyngeal carcinoma tissue section showing dark nuclear staining (arrows). (X40 objective)