Table I.
Primer sequences for detection of PRNCR1 by PCR-RFLP.
| PRNCR1 SNPs | PCR primers (5′→3′) | Restriction enzyme | Fragment length (bp) |
|---|---|---|---|
| rs13252298 A>G | F: CAGCACTTGCTGTCTTCTCAGATACgAT | EcoRV | AA:196+28 |
| R: TACTCCCCAATCTCTGGTCTTACCT | AG:224+196+28 | ||
| GG:224 | |||
| rs1456315 G>A | F: TTGCATTACCTCAACTAAGCCAAG | AccI | GG:213+30 |
| R: GGATGAAGAACTGAGGTTGCTAATAAGTC | GA:243+213+30 | ||
| AA:243 | |||
| rs7841060 T>G | F: CACCAATCCCAGAGCCATTTTGT | RsaI | TT:225 |
| R: CATTTCTCAGGTAGACCATGAACCTCGTA | TG:225+197+28 | ||
| GG:197+28 | |||
| rs7007694 T>C | F: GCGAATGCCATTTGTTTGGACG | BstuI | TT:222 |
| R: CCTCCAAAGAGAAGAACGGCT | TC:222+200+22 | ||
| CC:200+22 |
PRNCR1, prostate cancer-associated non-coding RNA 1; PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; SNP, single-nucleotide polymorphism; F, forward; R, reverse.