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. 2017 Dec 21;36:188. doi: 10.1186/s13046-017-0659-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Interaction of TIP60-eGFP with UHRF1-mCherry evidenced by FRET-FLIM. a 25 μm × 25 μm FLIM images of HeLa cells transfected with TIP60eGFP (i) or co-transfected with TIP60-eGFP and UHRF1-mCherry (ii) and lifetime distribution curve (iii). Color coded images indicate the fluorescence lifetime of TIP60-eGFP at each pixel. Color scale codes for lifetimes ranging from 1.8 ns (red) to 2.8 ns (blue). b Fluorescence lifetimes in TIP60-eGFP () and TIP60-eGFP + UHRF1-mCherry co-transfected cells (). Values are means ± SEM from five independent experiments. For statistical analysis, a Student’s t-test was performed (*** P < 0.001)

Inline graphic — Interaction of TIP60-eGFP with UHRF1-mCherry evidenced by FRET-FLIM. a 25 μm × 25 μm FLIM images of HeLa cells transfected with TIP60eGFP (i) or co-transfected with TIP60-eGFP and UHRF1-mCherry (ii) and lifetime distribution curve (iii). Color coded images indicate the fluorescence lifetime of TIP60-eGFP at each pixel. Color scale codes for lifetimes ranging from 1.8 ns (red) to 2.8 ns (blue). b Fluorescence lifetimes in TIP60-eGFP () and TIP60-eGFP + UHRF1-mCherry co-transfected cells (). Values are means ± SEM from five independent experiments. For statistical analysis, a Student’s t-test was performed (*** P < 0.001)