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. 2017 Dec 21;36:188. doi: 10.1186/s13046-017-0659-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Interaction between TIP60-eGFP and UHRF1-mCherry in S phase of cell cycle. a-c TIP60-eGFP interaction with UHRF1-mCherry in early, mid and late S phases of cell cycle, respectively. Confocal images of cells labeled with TIP60-eGFP, UHRF1-mCherry, EdU-Alexa 647 and merge, respectively (i - iv). The white bar indicates size of 5 μm. 25 μm × 25 μm FLIM images of HeLa cells transfected with TIP60-eGFP (v) or co-transfected with TIP60-eGFP and UHRF1-mCherry (vi) and lifetime distribution curves of the respected cells (vii). Color scale codes for lifetimes ranging from 1.8 ns (red) to 2.8 ns (blue). d Fluorescence lifetime distributions of TIP60-eGFP (), TIP60-eGFP EdU labeled cells (), total TIP60-eGFP + UHRF1-mCherry co-transfected cells () and co-transfected cells in S-phase of cell cycle (). Values are means ± SEM from five independent experiments. For statistical analysis, a Student’s t-test was performed (*** P < 0.001)

Inline graphic — Interaction between TIP60-eGFP and UHRF1-mCherry in S phase of cell cycle. a-c TIP60-eGFP interaction with UHRF1-mCherry in early, mid and late S phases of cell cycle, respectively. Confocal images of cells labeled with TIP60-eGFP, UHRF1-mCherry, EdU-Alexa 647 and merge, respectively (i - iv). The white bar indicates size of 5 μm. 25 μm × 25 μm FLIM images of HeLa cells transfected with TIP60-eGFP (v) or co-transfected with TIP60-eGFP and UHRF1-mCherry (vi) and lifetime distribution curves of the respected cells (vii). Color scale codes for lifetimes ranging from 1.8 ns (red) to 2.8 ns (blue). d Fluorescence lifetime distributions of TIP60-eGFP (), TIP60-eGFP EdU labeled cells (), total TIP60-eGFP + UHRF1-mCherry co-transfected cells () and co-transfected cells in S-phase of cell cycle (). Values are means ± SEM from five independent experiments. For statistical analysis, a Student’s t-test was performed (*** P < 0.001)