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. 2017 Dec 21;48:89. doi: 10.1186/s13567-017-0494-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Protection in pigs. A Survival curve of swine after challenge with SS2. Groups of ten pigs were orally immunized twice at 3-week intervals with indicated strains and challenged intravenously with 10⁸ CFU of S. suis 2 at 2 weeks after the 2^nd immunization. (B) Clinical symptom of leg and histopathology in the brain of immunized pigs challenged with SS2. (C) Western blots analysis the ability of sera from the pigs vaccinated with rSC0016 or rSC0018 containing pS-SaoA plasmid to recognize SaoA protein of SS2 and SS7. PB: Post-boost serum of pigs. (D) Rectal temperature of immunized piglets after challenged intravenously with 10¹⁰ CFU of SS7. The rectal temperatures were measured at 0, 1, 2, 3, 4, 5, 6, and 7 days after challenge. The experiment was performed twice.