Table 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics or genotype | Source or references |
|---|---|---|
| E. coli strains | ||
| BL21 | F− pT hsdSB(rB− mB− al dcm (DE3) | Invitrogen |
| χ7213 | thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 asdA4 recA1 RP4 2-Tc::Mu pir; Km r | Dr Curtiss gift |
| Salmonella Choleraesuis | ||
| C78-3 | Wild type, virulent, CVCC79103 | [65] |
| C500 | Salmonella Choleraesuis vaccine strain attenuated by chemical mutation, CVCC79500 | [37] |
| rSC0010 | ΔPcrp527::TT araC PBAD crp Δpmi-2426 ΔrelA199::araC PBAD lacI TT | [34] |
| rSC0011 | ΔPcrp527::TT araC PBAD crp Δpmi-2426 ΔrelA199::araC PBAD lacI TT ΔasdA33 | [34] |
| rSC0013 | rSC0010 ΔsopB1686 | This work |
| rSC0016 | rSC0013 ΔasdA33 | This work |
| rSC0017 | C78-3 ΔsopB1686 | This work |
| rSC0018 | C500 ΔasdA33 | This work |
| Streptococcus suis serotype 2 | Wild-type, virulent, CVCC3928 | Lab stock |
| Streptococcus suis serotype 7 | Wild-type isolated in field | Lab stock |
| Plasmids | ||
| pYA3493 | Plasmid Asd + ; pBR ori, β-lactamase signal sequence-based periplasmic secretion plasmid | [46] |
| pET28a | expression vector, T7 promoter; Kmr | Novagen |
| pRE112 | oriT oriV sacB CmR | [34] |
| pS006 | suicide vector for ΔsopB1686 | This work |
| pYA3736 | suicide vector for ΔasdA33 | pRE112 |
| pET28a-saoA | expression vector for purification of Hig-tag SaoA, T7 promoter, Km+ | pET28a |
| pS-SaoA | pYA3493 with SaoA | pYA3493 |