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. 2017 Dec 21;10:617. doi: 10.1186/s13071-017-2576-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunoblotting assays. A molecular weight marker (Lane a), 20 μg L. infantum SLA (Lane b) and 10 μg rLiHyp (Lanes c, d) were electrophoresed on a 20% SDS-PAGE and blotted onto nitrocellulose membranes. Blots were incubated with pools of sera from B1 phage-vaccinated mice (Lanes b and c) or from naive mice (lane d), and were revealed by adding chloronaphtol, diaminobenzidine, and H₂O₂. A scan from the blots is shown here. The black arrow indicates the rLiHyp protein (~28.0 kDa)