Fig. 7.

Involvement of CD4⁺ or CD8⁺ T cells in IFN-γ production. Spleen cells of mice that were immunized with B1 or D11 clones, and later infected, were in vitro stimulated with the respective clones (1 × 10¹⁰ phages) or with SLA (25 μg/ml) for 48 h at 37 °C in 5% CO₂. After, these were maintained in the absence (control) or incubated in the presence of monoclonal antibodies against mouse IL-12, CD4⁺ or CD8⁺ (5 μg/ml each). The IFN-γ production in the supernatants was evaluated, and the results are shown here. Bars represent the mean ± standard deviation. ***P < 0.0001 (a statistically significant difference in relation to the control group)