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. 2017 Dec 21;10:617. doi: 10.1186/s13071-017-2576-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Phage and parasite-specific humoral response before and after infection. BALB/c mice received saline or were immunized with Wild-type (WT), Random, B1 or D11 clones. Thirty days after the last immunization, sera samples were collected (Before). In addition, remaining animals were challenged with L. infantum promastigotes and followed up for 60 days, at which time sera samples were collected (After). The anti-phage and anti-parasite IgG2a and IgG1 isotype antibody levels were obtained, and the ratios between IgG2a and IgG1 results were calculated and are shown here. Bars represent the mean ± standard deviation. ***P < 0.0001 (a statistically significant difference in relation to the saline, WT and Random groups)