Table 1.
Biophysical antibody profiles
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Fragment | Fab | Fc | ||||||
| Feature | Antigen target | Epitope target | Antigen affinity | Isotype | Subclass | Glycans | FcR/C’ binding | FcR affinity |
| Examples of measurements | gp120 gp41 p24 |
Scaffolds -V1V2 -SOSIP -linear peptides |
Dissociation constant (k-off) |
IgA IgM IgG |
IgG1 IgG2 IgG3 IgG4 IgA1 IgA2 |
~30 glycan combinations Eg. Fucose, bisecting GLNAc, Galactose, Sialic Acid |
C1q MBL FcγRI FcγRIIa FcγRIIb FcγRIIIa FcγRIIIb |
Equilibrium constants (KD) |
| Examples of Assays | Multiplex ELISAs |
Multiplex ELISAs ICS |
SPR Chaotrope |
Multiplex ELISAs |
Multiplex ELISAs |
Mass Spec HPLC CE Multiplex |
ELISA Multiplex |
SPR |
| References | [104] | [31, 40] | [105] | [104] | [104] | [53, 87, 106] | [44, 87] | [105, 107] |