Table 2. Next-generation primers used for PCR amplification of samples of soil microbial communities.
Marker | Size | Primer’s name | Sequence | Reference |
---|---|---|---|---|
16S rRNA (bacteria) | 460 bp | Bakt_341F | 5′-CCTACGGGNGGCWGCAG-3′ | Herlemann et al. (2011) |
Bakt_805R | 5′-GACTACHVGGGTATCTAATCC-3′ | |||
16S rRNA (archaea) | 570 bp | 340F | 5′-CCCTAYGGGGYGCASCAG-3′ | Gantner et al. (2011) |
915R | 5′-GTGCTCCCCCGCCAATTCCT-3′ | Stahl & Amann (1991) | ||
ITS (fungi) | 330 bp | ITS3 | 5′-GCATCGATGAAGAACGCAGC-3′ | White et al. (1990) |
ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | |||
18S rRNA (other eukaryotes) | 165 bp | 1380F | 5′-CCCTGCCHTTTGTACACAC-3′ | Amaral-Zettler et al. (2009) |
1510R | 5′-CCTTCYGCAGGTTCACCTAC-3′ |