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. 2017 Dec 19;5:e4178. doi: 10.7717/peerj.4178

Table 2. Next-generation primers used for PCR amplification of samples of soil microbial communities.

Marker Size Primer’s name Sequence Reference
16S rRNA (bacteria) 460 bp Bakt_341F 5′-CCTACGGGNGGCWGCAG-3′ Herlemann et al. (2011)
Bakt_805R 5′-GACTACHVGGGTATCTAATCC-3′
16S rRNA (archaea) 570 bp 340F 5′-CCCTAYGGGGYGCASCAG-3′ Gantner et al. (2011)
915R 5′-GTGCTCCCCCGCCAATTCCT-3′ Stahl & Amann (1991)
ITS (fungi) 330 bp ITS3 5′-GCATCGATGAAGAACGCAGC-3′ White et al. (1990)
ITS4 5′-TCCTCCGCTTATTGATATGC-3′
18S rRNA (other eukaryotes) 165 bp 1380F 5′-CCCTGCCHTTTGTACACAC-3′ Amaral-Zettler et al. (2009)
1510R 5′-CCTTCYGCAGGTTCACCTAC-3′