Fig. 1.

The effect of shoot age on the transient expression efficiency of roses. The fresh cut adventitious buds were transferred on proliferation medium and cultured for 3 weeks, and then transferred to rooting medium and grown for another 3 weeks to develop roots, the seedlings with roots were then planted in soil. Sub-culture times indicated in the figure refer to the days from the first transfer until LUC analysis. a Bright-field, b dark-field, c intensity of LUC bioluminescence quantified using Andor Solis image analysis software. Data are mean ± SEM of five biological replicates each with three technical repeats, 20 shoots were used in each technical repeat. Asterisks denote a significant P < 0.05