Fig. 5.
KV7 channel pharmacological activation with ML213 leads to an inhibition of nerve-evoked contractions induced by 10- and 20-Hz EFS in DSM isolated strips. (A) A representative isometric DSM tension recording demonstrating the concentration-dependent inhibition of 10 Hz EFS-induced contractions by ML213 (0.1–30 µM) in DSM isolated strips. (B) A representative isometric DSM tension recording illustrating the concentration-dependent inhibition of 20-Hz EFS-induced contractions by ML213 (0.1–30 µM) in DSM isolated strips. Cumulative concentration-response curves for ML213 on DSM EFS-induced contraction amplitude (C) and muscle force (D) (n = 7, N = 6 for 10 Hz; n = 6, N = 6 for 20 Hz; *P < 0.05 for 10 versus 20 Hz; two-tailed paired Student’s t-tests). See Table 1 for potency and maximum efficacy values and Supplemental Fig. 5 for illustration of summary concentration-responses, data for which are shown as the mean ± S.D.