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. 2017 Dec 19;13:11–27. doi: 10.2147/COPD.S144136

Figure 2.

Figure 2

Generation of a human-specific BPIFA1 antibody.

Notes: An affinity-purified, peptide-specific, rabbit polyclonal antibody against human BPIFA1 was generated and validated by Western blotting using apical secretions from air–liquid interface cultures of human bronchial epithelial cells. The BPIFA1 antibody was used to probe two air–liquid interface wash samples (A84 and A42) at a final dilution of 1:200. The black arrows indicate the positions of the molecular mass markers.

Abbreviation: BPIF protein, bactericidal/permeability-increasing fold-containing protein.