Figure 4.

ORMDL3 expression.

Notes: (A) EOS and PBMCs were isolated from six blood donors, mRNA was purified, and gene expression was measured by Q-PCR. Box plots of ORMDL3 gene expression normalized to b-actin and shown as fold inductions relative to the control (monocyte population) from the same donor. Boxes denote the median and 25th to 75th percentiles with whiskers extending to the farthest points within 1.5 times the box height. (B) EOS and PBMC cell lysates were prepared from a representative donor and protein expression of ORMDL measured by Western blot.

Abbreviations: EOS, eosinophils; PBMCs, peripheral blood mononuclear cells.