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. 2017 Jun 19;9(27):22321–22331. doi: 10.1021/acsami.7b07166

Figure 9.

Figure 9

(a) Fluorescence intensity (normalized to that of alginate-based beads in the absence of autoinducers) of E. coli pLuxR-GFP in alginate-based beads exposed to 1.0 × 10–6 mol/L 3OC12HSL. (b) Fluorescence microscopy image of beads loaded with E. coli pLuxR-GFP in the presence of 1.0 × 10–6 mol/L 3OC12HSL after 3 h incubation. Normalized GFP expression of E. coli pLuxR-GFP in the presence of 1.0 × 10–5, 1.0 × 10–6, and 1.0 × 10–7 mol/L 3OC12HSL (fluorescence intensity divided by OD600 and normalized to that of E. coli pLuxR-GFP culture in the absence of 3OC12HSL which was set to “1” as a reference) over time: (c) in LB medium; (d) inside of alginate-MA beads.