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. Author manuscript; available in PMC: 2017 Dec 22.
Published in final edited form as: Protein Expr Purif. 2017 Jan 18;132:60–67. doi: 10.1016/j.pep.2017.01.008

Figure 1.

Figure 1

Schematic of CYP725A4 (grey) and CPR (cyan) inserted into the nanodisc (lipids are purple, phosphate atoms are yellow, and membrane scaffold protein is shown in blue). When NADPH is given to the system, the electrons are used in conjunction with molecular oxygen to convert taxadiene to taxadiene-5α-ol or OCT.