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. Author manuscript; available in PMC: 2017 Dec 22.
Published in final edited form as: Protein Expr Purif. 2017 Jan 18;132:60–67. doi: 10.1016/j.pep.2017.01.008

Figure 3. Characterization of CYP725A4.

Figure 3

(A) UV-Vis spectra of CYP725A4-17-α-L-CPR. The peak at 417 nm is characteristic of Soret band of heme proteins. The shoulder at 456nm is due to the flavin cofactors of CPR that is linked to the CYP. (B) Size exclusion chromatogram of CYP725A4-17α-L-CPR Nanodisc mixture. The peak at 16.5 mL is indicative of CYP725A4-17α-L-CPR-nanodisc.