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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2017 Nov 10;338:30–42. doi: 10.1016/j.taap.2017.11.006

Figure 1. Effects of DCVC on apoptosis in HTR-8/SVneo cells.

Figure 1

Cells were treated for 12 h or 24 h with 0 (no treatment control), 10, 20, 50, or 100 μM DCVC. A) Annexin V-FITC and propidium iodide fluorescence indicators were used to quantify apoptosis using flow cytometry. The dot plots are representative of each experiment at 24 h. B) Graphical representation of total apoptosis (early+late apoptosis). Bars represent means±SEM. Data were analyzed by two -way ANOVA (interaction between time and treatment, P=0.001) with posthoc Tukey multiple comparisons. #Significantly different compared to all 12 h time points (P<0.0022). &Significantly different compared to control and 10 μM DCVC at 24 h time point (P<0.0088). +Significantly different compared to control, 10 and 20 μM DCVC at 24 h time point (P<0.0001). *Significantly different compared to 50 μM DCVC at 24 h time (P=0.005). N=4 independent experiments for 12 h and N=5 independent experiments for 24 h. All experiments were performed in triplicate. Camptothecin (4 μM) was included as a positive control and increased total apoptosis 32.89% +/−15.23% at 12 h and 39.11% +/− 6.36 at 24 h.