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. 2017 Nov 1;67(1):85–97. doi: 10.2337/db17-0367

Figure 6.

Figure 6

Conditional inactivation of Baf60a and Baf60c in skeletal muscle increases metabolic rate in ACKO mice. A and B: Oxygen consumption rate (VO2) (A) and energy expenditure (EE) (B) in control (Ctrl) and ACKO mice fed with HFD for 1 week (n = 8). C: qPCR analysis of BAT gene expression in mice fed with standard chow (left, n = 4–5) or HFD for 12 weeks (right, n = 5–11). D: Immunoblots of BAT total protein lysates from mice as described in C. E: qPCR analysis of quadriceps gene expression in HFD-fed mice (n = 6–7). F: qPCR analysis of quadriceps gene expression in chow-fed old mice (n = 6–7). G: Immunoblots of total muscle protein lysates from the HFD-fed (left) and aging (right) cohorts. H: Left, lactate production in ex vivo cultured EDL muscle treated with vehicle (Veh) or dantrolene (DAN) (100 μmol/L) for 3 h. Right, DAN-inhibitable lactate production rate calculated from data on the left. Data in A–C, E, F, and H represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.