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. 2017 Nov 1;67(1):85–97. doi: 10.2337/db17-0367

Figure 7.

Figure 7

Baf60 deficiency enhances the expression and secretion of IGF2. A: Heat map representation of Baf60-regulated genes encoding secreted factors. B: qPCR analysis of gene expression in quadriceps muscle from AKO (n = 3), CKO (n = 6), ACKO (n = 4–5) mice and their littermate controls (Ctrl) on standard chow diet. C: IGF2 secretion in ex vivo cultured soleus (left) or plantaris (right) muscles. D: Plasma IGF2 concentration in chow-fed control and ACKO mice (n = 4–5). E: qPCR analysis of quadriceps gene expression in HFD-fed mice (left, n = 5–11) and plasma IGF2 concentrations in HFD-fed mice (right, n = 7–9). F: qPCR analysis of quadriceps gene expression (left) and plasma IGF2 levels (right) in old control and ACKO mice (n = 6–7). G: ChIP assays on control and ACKO muscle nuclei using antibodies against histone 3 acetylation (AcH3) and H3K9 demethylation (H3K9me2). Location of primers on the proximal regions of three IGF2 promoters is shown. H: Schematic model demonstrating the regulation of exercise preformation and systemic energy homeostasis by Baf60a and Baf60c. Data in B–G represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.