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. 2017 Aug 29;67(1):46–57. doi: 10.2337/db17-0546

Figure 6.

Figure 6

SCP4−/− neonates display hypoglycemia. A: Blood glucose levels are decreased in SCP4−/− mice. E18.5 WT (n = 19), SCP4+/− (n = 13), and SCP4−/− (n = 12) mice were starved for 12 h. Blood from animal tails was collected for glucose measurement. B: Glucose but not pyruvate injection can extend the survival time of SCP4−/− mice. SCP4−/− mice at E18.5 were injected with saline alone (n = 5), glucose-containing saline (n = 4), or pyruvate-containing saline (n = 4) every 6–8 h. The survival status of mice was monitored every hour. C: Gluconeogenesis rate is decreased in adult SCP4+/− mice. Six-week-old heterozygous SCP4+/− and WT littermates were starved for 48 h and then injected with sodium pyruvate. Their blood glucose levels were measured at indicated time points. D: There is no significant difference in the mRNA levels of insulin and glucagon between SCP4−/− and WT littermates. Total RNAs were extracted from the pancreas of E18.5 SCP4−/− and WT mice after 12-h starvation and subjected to qRT-PCR to examine the expression levels of insulin and glucagon. Data are shown as means ± SEM; n = 6. E: Autophagy is not affected in SCP4−/− mice. Autophagy marker LC-3 I/II was determined by IB in the liver lysates of E18.5 SCP4−/− and WT mice after 12 h starvation. *P < 0.05; ***P < 0.001.