Fig 8. Forced expression of IL-7R and PDL-1 deficiency acts in synergy to promote cytotoxic response to self antigen.

A: 4x 10⁶ or 0.5x 10⁶ w.t. OT-I T cells were injected into w.t. or PDL-1-/- RIP-mOVA mice (n = 5–9 in each group). The recipients were monitored for diabetes by urine analysis. *** p< 0.001 by log-rank test. B: 0.5 x 10⁶ w.t or IL-7R Tg OT-I T cells were transferred into PDL-1-/- RIP-mOVA mice (n = 13–14 in each group). The recipients were monitored for diabetes by urine analysis. *** p< 0.001 by log-rank test. Representative H&E staining of the recipients’ pancreata are shown. C: 5 x 10⁶ w.t. or IL-7R Tg OT-I T cells were transferred into w.t. or PDL1-/- RIP-sOVA mice (n = 5 in each group). Pancreata were collected from the recipients at day 14 after transfer. Representative H&E staining of the pancreata from the PDL1 -/- RIP-sOVA recipients are shown. Right: summary of insulitis (10 islets from each pancreas were scored as Fig 3B). ** p<0.01 by Mann-Whitney U test.