Fig 7. The interaction between NUP85 and MED18.

(A) Split luciferase complementation assay in Arabidopsis protoplasts showing the interaction between NUP85 and MED18. Empty Cluc and PYL1-Cluc were used as negative controls. Approximately 1×10⁴ protoplasts per sample were co-transformed with indicated plasmids. The split-LUC complementation assay was repeated three independent times with consistent results. (B) Co-immunoprecipitation assays in Arabidopsis protoplasts confirming the interaction between NUP85 and MED18. CDK8-HA was used as a control. (C) The relative gene expression of RD29A, COR15A and COR47 in wild type and med18 mutants under mock or ABA treatments. (D)The relative gene expression of RD29A, COR15A and COR47 in wild type and med18 mutants under mock or salt treatments (0.3 M NaCl). In C and D, values indicate means ± SD (n = 3). Significance between the mean values were analyzed with Student’s t test (* P< 0.05).