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. 2017 Dec 12;13(12):e1007121. doi: 10.1371/journal.pgen.1007121

Fig 4. SYGL-1 and LST-1 tumor formation relies on FBF.

Fig 4

(A-I) Epistasis tests using sygl-1(ubiq) or lst-1(ubiq) transgenes. All images are dissected young adult gonads stained with sperm marker SP56 (red) and DAPI (cyan). (A-C) Epistasis with glp-1. (A) GSC defect in glp-1(q46) null: the few GSCs in L1 larvae differentiate as sperm [14]. (B and C) Germline tumor in sygl-1(ubiq); glp-1(q46) null and lst-1(ubiq); glp-1(q46) null. (D-F) Epistasis with lst-1 sygl-1. (D) GSC defect in lst-1(ok814) sygl-1(tm5040) double mutant is indistinguishable from that of glp-1 null [18]. (E and F) Germline tumor in lst-1(ok814) sygl-1(tm5040); sygl-1(ubiq) and in lst-1(ok814) sygl-1(tm5040); lst-1(ubiq). (G-I) Epistasis test with fbf-1 fbf-2. GSC defect in fbf-1(ok91) fbf-2(q704) double mutant: GSCs made in larvae but not maintained past late L4 when all differentiate as sperm at 15°C and 20°C [15]. At 25°C, a small number of GSCs is maintained in adults [40]. (H and I) GSC defect similar to that of fbf-1 fbf-2 double mutant in fbf-1(ok91) fbf-2(q704) sygl-1(ubiq) and fbf-1(ok91) fbf-2(q704) lst-1(ubiq). See S5 Fig for confirmation that SYGL-1 and LST-1 are expressed and functional in these strains, and for characterization of these strains at 25°C. Conventions as in Fig 1E–1J; scale bar is 20 μm. In all strains, sygl-1(ubiq) is qSi235[Pmex-5::3xFLAG::sygl-1::tbb-2 3’end] and lst-1(ubiq) is qSi267[Pmex-5:: lst-1::3xFLAG::tbb-2 3’end]. (J) Summary of epistasis results. (K) Revised genetic model for GSC regulation. See text for further explanation.