Figure 1.
Effect of a high-fat diet and intermittent calorie restriction on tumor growth and cancer stem cells. (A) Protocol for intermittent calorie restriction (ICR) with control and high-fat diets. CT26 cells (1×107) were implanted into the scapular subcutaneous tissues of BALB/c mice. Mice were subjected to a 24-h fasting period once each week for 4 weeks. Tumors were excised and examined on day 29. (B) Mouse body weight. (C) Tumor weight. (D) Number of nodal metastases. (E) CD133 immunostaining of tumors from mice in groups F (high-fat diet) and FI (high-fat diet with ICR). CD133-positive cells are indicated by red arrows. Scale bar, 50 μm. (F) Number of CD133+ cells in tumors of mice in each group. Group C, controls not subjected to ICR; group CI, controls subjected to ICR.