Figure 3.

Effect of an elaidic acid (EA)-enriched diet with intermittent calorie restriction (ICR) on tumor growth and cancer stem cells. (A) Protocol for ICR with a control and EA-enriched diet. CT26 cells (1×10⁷) were implanted into the scapular subcutaneous tissues of BALB/c mice. Mice were subjected to a 24-h fasting period once each week for 4 weeks. Tumors were excised and examined on day 29. (B) Mouse body weight. (C) Tumor weight. (D) MIB1 index. (E) Percentage of nucleostamin-positive (NS⁺) cells. (F) Percentage of CD133⁺ cells. Error bars indicate the means ± standard deviations. Group C, controls not subjected to ICR; group CI, controls subjected to ICR; group E, mice given EA-rich diet not subjected to ICR; group EI, mice given EA-rich diet subjected to ICR.