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. 2017 Dec 18;52(2):413–423. doi: 10.3892/ijo.2017.4229

Figure 4.

Figure 4

Effect of an EA-enriched diet with intermittent calorie restriction (ICR) on cancer metastasis. (A) Temporal changes in food intake and the number of nucleostamin-positive (NS+) and CD133+ cells in group E (EA-rich diet without ICR) tumors. (B) Temporal changes in food intake and the number of NS+ and CD133+ cells in group EI (EA-rich diet with ICR) tumors. Food calorie intake was standardized to that on day 1 for group E, which was set as 100%. Positivity of NS or CD133 was calculated as the percentage of positively stained cells among 200 cells observed. (C) Time-course analysis of NS mRNA levels in the blood. (D) Micrometastasis in the lung on day 29. Cancer cells were detected by ICG-labeled anti-EGFR antibody and measured using an in vivo imager. Error bars indicate the means ± standard deviations. Group C, controls not subjected to ICR; group CI, controls subjected to ICR; group E, mice given EA-rich diet not subjected to ICR; group EI, mice given EA-rich diet subjected to ICR.