Table III.
Effects of the inhibitors of IGF-1R and HIF-1α on energy metabolism in CT26 cells.
| Glucose concentration (mg/dl) | 150 | 150 | P-valuea | 150 | P-valuea |
|---|---|---|---|---|---|
| Inhibitor | None | IGF-1R | HIF-1α | ||
| MIB1 positivity (%) | 71±6.5 | 45±4 | <0.01 | 42±3.1 | <0.01 |
| NS positivity (%) | 61±6.3 | 42±4.4 | <0.05 | 40±3.5 | <0.01 |
| O2 consumption (kpiu)b | 4.67±0.298 | 7.44±0.752 | <0.001 | 7.58±0.788 | <0.001 |
| Lactate (pM) | 7.9±0.84 | 2.2±0.24 | <0.001 | 2.0±0.25 | <0.001 |
| ROS (%)c | 64±7 | 99±10 | <0.01 | 103±11 | <0.01 |
a
Statistical significance was calculated by a two-tailed Student's t-test
b
measured by MitoXpress dye staining; kpiu, kilo phosphorescence intensity
c
reactive oxygen species levels were measured by CellROX Green dye staining. Fluorescence intensity is standardized by that of control diet-starvation group, which is set to 100%. Anti-Kie-67 protein antibody clone MIB1; NS, nucleostemin; ROS, reactive oxygen species; IGF-1R, insulin-like growth factor-1 receptor; HIF-1α, hypoxia-inducible factor-1α.