Fig. 8.

Schematic representation for cloning of the bta-miR-2898 target region in pMIR-GFP expression vector. a Identified target sites for bta-miR-2898 at 3′ UTR region of bovine HSPB8 (Accession Number: NM001014955); bases with capital letters are the target sites where miR2898 can interact, and highlighted bases are the sites chosen for designing primers to amplify the target regions. b Strategy for construction of pMIR-GFP target, where the bta-mir 2898 target sites at 3′ UTR region of bovine HSPB8 was cloned in pMIR-GFP expression vector. Target region was fused at 3′ UTR region of GFP. HindIII and SpeI are the linkers added at the 5′ region of forward and reverse primers, respectively, to clone at multiple cloning sites (MCS) region of the expression vector