Fig. 9.

pMIR–GFP reporter assay in bovine PBMC (heat shocked) for analysis of the expression of GFP after cloning of bta-mir 2898 target region of bovine HSP gene at 3′ UTR region. Upper panel depicted the experimental design and lower panel showed the level of GFP expression in different groups. a Non-heat shock group and transfected with pMIR–GFP target construct. b Heat shock group and transfected with pMIR–GFP target construct. c Heat shock group and co-transfected with pMIR–GFP target construct and anti-bta–mir 2898