Fig. 2.

CO accumulates to high levels in the cop1-4 background regardless of FKF1. a CO accumulation at ZT15 in 10-day-old seedlings under LD. b CO accumulation in various plants. Plants were grown for 10 days under LD and harvested every 4 h over the course of a day. Nuclear protein-enriched fractions were immunoblotted using an anti-CO antibody (α-CO) to measure CO levels and an anti-H3 antibody (α-H3) for a loading control. Data are means ± s.d. from at least three biological repeats. c Relative abundance of CO mRNA during a day in 10-day-old seedlings. d Native CO mRNA levels during a day in 35S::CO-GFP transgenic plants. To detect the native CO mRNA, a specific primer was designed from the 3′-UTR region of the CO mRNA sequence. c, d For RT-qPCR, the relative expression level of each gene was normalized to the mRNA level of ACTIN (AT3G18780) as a loading control. Data are means ± s.d. from three biological replicates