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. 2017 Dec 22;8:2259. doi: 10.1038/s41467-017-02476-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — COP1 variants that are unable to homo-dimerize are non-functional. a Mutation sites in the CLS motif of COP1. b COP1^L170A forms dimers poorly when compared with COP1^WT or COP1^L105A in N. benthamiana. c FKF1 interacts more weakly with COP1^L170A (poor dimer formation) than COP1^L105A (normal dimer formation) in N. benthamiana. d Various transgenic plants at bolting under LD and SD. Scale bars, 2 cm. e Flowering times of the plants in d. The number of rosette leaves at bolting represents the flowering time of each genotype. Data are means ± s.d. of 20 plants. f CO accumulation of each genotype in d. Ten-day-old plants were harvested at ZT15 (1 h before dark) in LD. g Hypocotyl elongation of plants in d grown for 5 days in complete darkness. Scale bars, 1 cm. h Hypocotyl length of transgenic plants in darkness. Data are means ± s.d. of 20 plants. i HY5 accumulation in various transgenic plants