Figure 4.

Phylogenetic tree of cyclotide precursors. The tree contains 92 precursor sequences in total, with two representative sequences from each of the 46 molecular species. Except from the nine molecular species (i.e., GA2, GP1, NS2, NS5, NL2, HS4, PN1, QD1, and YY1), all other 37 molecular species (80%, 37/46) are grouped as one monophyletic clade (posterior probability (pp) ≥ 0.75). Also, except from four molecular series (i.e., FA, HF, HS, and YA), the molecular species belong to other nine molecular series are monophyletic (pp ≥ 0.97). In the phylogenetic tree, DI1 is a single exception from the lineage classification based on sequence signature. DI1 is grouped into Möbius lineage in the phylogenetic tree (pp ≥ 0.70), even though it is classified as bracelet lineage based on the sequence signature.