Table 2.

Violaceae species sampled for the analysis of precursor sequences.

Genus	Section	Species
Viola	Plagiostigma	V. mandshurica W.Becker [37]a, V. albida Palib. var. takahashii (Nakai) Kitag. [29]a, V. verecunda A.Gray [23]a, V. baoshanensis W.S.Shu, W.Liu & C.Y.Lan [44]b
	Viola	V. odorata L. [5]c, V. uliginosa Bess. [18]d V. acuminata Ledeb. [23]a V. adunca Sm. [2]e
	Melanium	V. tricolor L. [49]f
	Chamaemelanium	V. orientalis W.Becker [26]a, V. biflora L. [6]g, V. canadensis L. [19]h, V. pinetorum Greene [2]e
Gloeospermum		G. blakeanum (Standl.) Hekking. [5]i, G. pauciflorum Hekking. [4]i
Melicytus		M. ramiflorus J.R.Forst. & G.Forst. [14]j
Pigea		P. floribunda Lindl. (= Hybanthus floribundus (Lindl.) F.Muell.) [6]k

The numbers in parentheses after the species name show the number of precursor sequences derived from that species. Species names marked in bold were used for transcriptomic assay of the current study. Sections are indicated within Viola. Within sect. Plagiostigma, V. mandshurica, and V. albida var. takahashii belong in subsect. Patellares (Boiss.) Rouy & Foucaud, V. verecunda in subsect. Bilobatae (W.Becker) W.Becker and V. baoshanensis in subsect. Diffusae (W.Becker) Chang. Within sect. Viola, V. odorata belongs to subsect. Viola and V. acuminata, V. adunca, and V. uliginosa in subsect. Rostratae (W.Becker) W.Becker. No subsections are applicable to sect. Chamaemelanium.

^aTranscriptomes from the current study, V. mandshurica, V. albida var. takahashii, V. verecunda, V. acuminata and V. orientalis;

^bV. baoshanensis (Zhang et al., 2009, 2015b);

^cV. odorata (Dutton et al., 2004; Ireland et al., 2006);

^dV. uliginosa (Slazak et al., 2015);

^eV. pinetorum and V. adunca (Kaas and Craik, 2010);

^fV. tricolor (Mulvenna et al., 2005; Hellinger et al., 2015);

^gV. biflora (Herrmann et al., 2008);

^hTranscriptome data for V. canadensis were obtained from the 1kp project (www.onekp.com), and the cyclotide precursor sequences were determined in this work;

ⁱG. blakeanum and G. pauciflorum (Burman et al., 2010);

^jM. ramiflorus (Trabi et al., 2009);

^kP. floribunda (as H. floribundus) (Simonsen et al., 2005).