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. 2017 Dec 18;10:422. doi: 10.3389/fnmol.2017.00422

Figure 3.

Figure 3

Effect of PNU-282987 on the expression levels of the GABA receptor subunit GABAAα1 in the retina. (A) Immunoblot analysis of GABAAα1 in the retinas of control and glaucomatous rats before and after PNU-282987 injection. Full-length blots are presented in Supplementary Figure S1. (B) Quantitative analysis of GABAAα1 expression. (C) Immunohistochemical analysis of the rat retina with antibodies against GABAAα1 labeled in ganglion cell layer (GCL; arrow) and outer plexiform layer (OPL; arrow) in the normal, glaucoma and glaucoma + PNU-282987 conditions. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer. Scale bar: 15 μm. The data are presented as the mean ± standard error of four samples for each experiment. *p < 0.05, **p < 0.01 (one-way analysis of variance).