Table 1.
Nucleotide sequences of primers used in this study.
| Primer name | Sequence (5′ to 3′) | Size of product (bp) | Reference |
|---|---|---|---|
| Sequencing | |||
| pmrA-F | CGCAGGATAATCTGTTCTCCA | 808 | This study |
| pmrA-R | GGTCCAGGTTTCAGTTGCAA | ||
| pmrB-F1 | GCGAAAAGATTGGCAAATCG | 659 | This study |
| pmrB-R1 | GGAAATGCTGGTGGTCATCTGA | ||
| pmrB-F2 | CCCTGAATCAGTTGGTTTC | 714 | This study |
| pmrB-R2 | ATCAATGGGTGCTGACGTT | ||
| mgrB-F | ACCACCTCAAAGAGAAGGCGTT | 347 | This study |
| mgrB-R | GGCGTGATTTTGACACGAACAC | ||
| phoP-F | GAGCGTCAGACTACTATCGA | 912 | This study |
| phoP-R | GTTTTCCCATCTCGCCAGCA | ||
| phoQ-F | CCACAGGACGTCATCACCA | 1,594 | This study |
| phoQ-R | GCAGGTGTCTGACAGGGATT | ||
| mcr-1-F | CGGTCAGTCCGTTTGTTC | 309 | Liu et al., 2016 |
| mcr-1-R | CTTGGTCGGTCTGTAGGG | ||
| mcr-2-F | TGTTGCTTGTGCCGATTGGA | 567 | Xavier et al., 2016 |
| mcr-2-R | AGATGGTATTGTTGGTTGCTG | ||
| mcr-3-F | TTGGCACTGTATTTTGCATTT | 542 | Yin et al., 2017 |
| mcr-3-R | TTAACGAAATTGGCTGGAACA | ||
| mcr-4-F | ATTGGGATAGTCGCCTTTTT | 487 | Carattoli et al., 2017 |
| mcr-4-R | TTACAGCCAGAATCATTATCA | ||
| RT-qPCR | |||
| pmrC-F | CTCTCGCCTCGTTCCTGAA | 140 | This study |
| pmrC-R | CGGAGTGGTGTCGAGGATA | ||
| pmrK-F | GGTGTATGCGATTGGCACCTA | 132 | This study |
| pmrK-R | AGCAGCACGTAGCCCAGTAT | ||
| rpsl-F | CCGTGGCGGTCGTGTTAAAGA | 109 | Cannatelli et al., 2013 |
| rpsl-R | GCCGTACTTGGAGCGAGCCTG | ||
| mgrB-ext-F | AAGGCGTTCATTCTACCACC | 253 | Cannatelli et al., 2013 |
| mgrB-ext-R | TTAAGAAGGCCGTGCTATCC | ||
| pmrB-ext-F | ACCTACGCGAAAAGATTGGC | 1,274 | Jayol et al., 2014 |
| pmrB-ext-R | GATGAGGATAGCGCCCATGC |