Table 2.
Phenotypic and genotypic characteristics of the studied K. pneumoniae strains.
| Isolate | Year, ward, and source of isolation | MIC (μg/ml) | mgrB sequence | pmrB sequence | Relative expression level |
|
|---|---|---|---|---|---|---|
| pmrK | pmrC | |||||
| KP13 | 2016-Neurology-Wound | 128 | Insertional inactivation by IS1-like (+120) | A246T | 3.83 ± 1.01 | –a |
| KP12 | 2016-Emergency-Blood | 64 | Insertional inactivation by IS1-like (+120) | A246T | 4.12 ± 0.05 | –a |
| KP16 | 2016-ICU-Wound | 128 | Insertional inactivation by IS1-like (+120) | A246T | 3.01 ± 0.02 | –a |
| KP01 | 2015-ICU-Blood | 128 | Premature termination by nonsense mutation at nt88 | L213M | 13.04 ± 0.5 | 1.87 ± 0.18 |
| KP04 | 2015-ICU-Blood | >128 | Premature termination by nonsense mutation at nt88 | L213M | 7.08 ± 0.25 | 3.5 ± 0.07 |
| KP07 | 2015-ICU-Tracheal aspirate | >128 | Premature termination by nonsense mutation at nt88 | L213M | 26.91 ± 2.68 | –a |
| KP08 | 2016-ICU-Blood | 128 | Premature termination by nonsense mutation at nt88 | L213M | 2.1 ± 0.77 | –a |
| KP09 | 2015-SICU-Tracheal aspirate | >128 | Premature termination by nonsense mutation at nt88 | L213M | 34.47 ± 1.0 | 9 ± 2.1 |
| KP10 | 2016-ICU-Tracheal aspirate | >128 | Premature termination by nonsense mutation at nt88 | L213M | 3.17 ± 0.03 | 3.66 ± 1.08 |
| KP18 | 2017-Internal medicine-Blood | >128 | Premature termination by nonsense mutation at nt88 | L213M | 1.97 ± 0.04 | –a |
| KP17 | 2016-Urology-Tracheal aspirate | 128 | Premature termination by nonsense mutation at nt117 | A246T | 3.96 ± 0.04 | –a |
| KP15 | 2016-ICU-Blood | 128 | premature termination by nonsense mutation at nt117 | A246T | 1.98 ± 0.12 | –a |
| KP02 | 2015-SICU-Urine | 32 | Insertional inactivation by IS5-like (+70) | R256G | 2.56 ± 0.14 | 3.26 ± 0.2 |
| KP11 | 2016-Internal medicine-Wound | 128 | Insertional inactivation by IS5-like (+70) | R256G | 77 ± 1.50 | 15.4 ± 3.9 |
| KP06 | 2016-Neurology-Tracheal aspirate | 64 | Insertional inactivation by IS1-like at promoter region (-60) | WT | 11.69 ± 2.59 | 9.15 ± 1.5 |
| KP03 | 2015-SICU-Blood | 128 | WTb | R256G | 64.36 ± 2.6 | 6.9 ± 0.83 |
| KP05 | 2015-Urology-Urine | 8 | WT | R256G | 24.3 ± 0.23 | 2.79 ± 0.15 |
| KP14 | 2016-Emergency-Urine | 128 | WT | R256G | 76.07 ± 6.2 | 7.06 ± 2.1 |
| KP51 | 2016-CCU-Urine | 0.5 | WT | WT | –a | –a |
| KP106 | 2016-Outpatient clinic-Urine | 0.5 | WT | WT | –a | –a |
| KP109 | 2016-Orthopedics-Urine | 0.25 | WT | WT | –a | –a |
| KP19 | 2017-ICU-Blood | 128 | WT | A246T | 2.2 ± 0.23 | 1.67 ± 0.33 |
| KP20 | 2017-ICU-Blood | 128 | WT | A246T | 2.5 ± 0.7 | 1.7 ± 0.53 |
aUnaltered expression levels compared to col-S isolates. bWT, wild-type.