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. 2017 Dec 18;8:2524. doi: 10.3389/fmicb.2017.02524

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Copyright © 2017 Gao, He, Quan, Jiang, Lin, Chen and Qu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of inclusion body proteins by SDS-PAGE and western blotting. M: protein marker; lanes 1 and 2: pET-NS4A-SLA-1^∗040101-β2m and pET-SLA-1^∗040101-β2m proteins after induction with IPTG in E. coli Rosetta; lane 3: pET-NS4A-SLA-1^∗040101-β2m protein before induction with IPTG; lanes 4 and 5: purified and refolded pET-NS4A-SLA-1^∗040101-β2m and pET-SLA-1^∗040101-β2m proteins; lanes 6 and 7: pET-NS4A-SLA-1^∗040101-β2m and pET-SLA-1^∗040101-β2m proteins visualized by western blotting. Arrows indicate expected position of recombinant proteins.