Table 2.

Nucleotides encoding viral CTL epitope peptides and the glycine-rich linker gene.

Virus	Protein	Sequence (5′-3′)a	Reference
		E N A L L V A L F
CSFV	NS4A	NS4A-P: TATG GAA AAC GCT CTG CTG GTT GCT CTG TTC GGTGGCGGTTCCGGCGGTGGCTCCGGCGGT G	Pauly et al., 1995
		NS4A-M: AATTC ACCGCCGGAGCCACCGCCGGAACCGCCACC GAACAGAGCAACCAGCAGAGCGTTTTC CA
		C L F A I L L A I
PRRSV	GP4	GP4-5P: TATG TGT CTT TTT GCC ATC CTA CTG GCA ATT GGTGGCGGTTCCGGCGGTGGCTCCGGCGGT G	Díaz et al., 2009
		GP4-5M: AATTC ACCGCCGGAGCCACCGCCGGAACCGCCACC AATTGCCAGTAGGATGGCAAAAAGACA CA
		N P E K P H F P L
	N	N-15P: TATG AAC CCG GAG AAG CCC CAT TTC CCT CTA GGTGGCGGTTCCGGCGGTGGCTCCGGCGGT G
		N-15M: AATTC ACCGCCGGAGCCACCGCCGGAACCGCCACC TAGAGGGAAATGGGGCTTCTCCGGGTT CA
		F S L P T Q H T V
		N-6P: TATG TTT AGT TTG CCG ACG CAA CAT ACT GTG GGTGGCGGTTCCGGCGGTGGCTCCGGCGGT G
		N-6M: AATTC ACCGCCGGAGCCACCGCCGGAACCGCCACC CACAGTATGTTGCGTCGGCAAACTAAA CA
		L A A L I C F V I R L A K N C
	GP5	G9-P: TATG CTG GCT GCG CTG ATT TGC TTT GTC ATT AGG CTT GCG AAG AAC TGC	Vashisht et al., 2008
		GGTGGCGGTTCCGGCGGTGGCTCCGGCGGT G
		G9-M: AATTC ACCGCCGGAGCCACCGCCGGAACCGCCACC GCAGTTCTTCGCAAGCCTAATGACAAAGC
		AAATCAGCGCAGCCAG CA

^aP and M indicate plus-sense and minus-sense sequences, respectively. Bold letters represent the sequences sticky to the ends of Nde I and EcoR I, respectively. Underlined part denotes the glycine-rich linker gene (Figure 1). The amino acid sequences of viral CTL epitope peptides are given above the plus-sense sequences.