Table 3.
Overlap PCR primers used for mutation of SLA-1∗04:01:01 and SLA-1∗13:01 molecules.
| Molecule | Name | Primer sequence (5′-3′)a | Mutant amino acid | Mutant molecule name |
|---|---|---|---|---|
| SLA-1∗04:01:01 | 04:01:01/66F | ATCGGGAGACGCGGAAAGTCAAGGAAAC | 66 (N→K) | SLA-1∗04:01:01/66 |
| 04:01:01/66R | GTTTCCTTGACTTTCCGCGTCTCCCGAT | |||
| 04:01:01/70F | AATGTCAAGGAAAACGCACAGACTTAC | 70 (T→N) | SLA-1∗04:01:01/70 | |
| 04:01:01/70R | GTAAGTCTGTGCGTTTTCCTTGACATT | |||
| 04:01:01/99F | TCCAGAGCATGTTTGGCTGCTACTTGGGA | 99 (Y→F) | SLA-1∗04:01:01/99 | |
| 04:01:01/99R | TCCCAAGTAGCAGCCAAACATGCTCTGGA | |||
| 04:01:01/151F | TGGGAGGCGGCCAATGAGGCGGAGCGTAGGA | 151 (D→N) | SLA-1∗04:01:01/151 | |
| 04:01:01/151R | TCCTACGCTCCGCCTCATTGGCCGCCTCCCA | |||
| 04:01:01/152F | GGCCGATGTGGCGGAGCGTAGGAGGAGCTA | 152 (E→V) | SLA-1∗04:01:01/152 | |
| 04:01:01/152R | TAGCTCCTCCTACGCTCCGCCACATCGGCC | |||
| 04:01:01/151/152F | TGGGAGGCGGCCAATGTGGCGGAGCGTAGGA | 151-152 (DE→NV) | SLA-1∗04:01:01/151/152 | |
| 04:01:01/151/152R | TCCTACGCTCCGCCACATTGGCCGCCTCCCA | |||
| SLA-1∗13:01 | 13:01/66F | ATGAGGAGACGCGGAATGTCAAGGACAA | 66 (K→N) | SLA-1∗13:01/66 |
| 13:01/66R | TTGTCCTTGACATTCCGCGTCTCCTCAT | |||
| 13:01/70F | AAAGTCAAGGACACCGCACAGACTTAC | 70 (N→T) | SLA-1∗13:01/70 | |
| 13:01/70R | GTAAGTCTGTGCGGTGTCCTTGACTTT | |||
| 13:01/99F | TCCAGAGCATGTACGGCTGCTACTTGGGA | 99 (F→Y) | SLA-1∗13:01/99 | |
| 13:01/99R | TCCCAAGTAGCAGCCGTACATGCTCTGGA | |||
| 13:01/151F | TGGGAGGCGGCCGATGTGGCGGAGCGTAGGA | 151 (N→D) | SLA-1∗13:01/151 | |
| 13:01/151R | TCCTACGCTCCGCCACATCGGCCGCCTCCCA | |||
| 13:01/152F | GGCCAATGAGGCGGAGCGTAGGAGGAGCTA | 152 (V→E) | SLA-1∗13:01/152 | |
| 13:01/152R | TAGCTCCTCCTACGCTCCGCCTCATTGGCC | |||
| 13:01/151/152F | TGGGAGGCGGCCGATGAGGCGGAGCGTAGGA | 151-152 (NV→DE) | SLA-1∗13:01/151/152 | |
| 13:01/151/152R | TCCTACGCTCCGCCTCATCGGCCGCCTCCCA |
aLetters in boldface type represent mutated nucleotides, which changed the corresponding amino acids.