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. 2017 Dec 18;7:514. doi: 10.3389/fcimb.2017.00514

Figure 7.

Figure 7

siRNA targeting reduces TLR4, MyD88, NFκB, and Caspase-1 expression in DCs. BMDCs were separately transfected with siRNA targeting TLR4, MyD88, NF-κB, or Caspase-1 for 48 h before stimulation with Pmp18.1 for 24 h. Protein extracts were subjected to SDS-PAGE and proteins were detected by Western immunoblotting analysis using rabbit monoclonal antibodies (mAb) to TLR 4 (D8L5W), MyD88 (D80F5), and NF-κB p65 (D14E12), and mouse mAb to Caspase-1 (14F468). The proteins bands were visualized using the ImageQuant LAS-4000 imaging system. Protein levels, normalized to GAPDH, were then quantified using Image J software. Each experiment was repeated at least twice. The results are from two independent experiments. Statistical analyses were performed using the Student's t-test. Statistically significant differences between rPmp18.1 stimulated and unstimulated DCs (Medium) and between siRNA-treated and scrambled siRNA-treated (S-siRNA) cells were evaluated using the Student's t-test and were considered to be statistically significant at (**p < 0.01).