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. 2017 Dec 22;7:18073. doi: 10.1038/s41598-017-18287-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Calculation of markers of cell type and subtypes. (a) Heatmap representing the markers of Oligo1, a subtype of oligodendrocytes (immature precursors, as defined in⁸). These markers are significantly more expressed in Oligo1 as they present yellow squares in all the other cell subtype (DESeq 2 corrected p-values). (b) Histogram (mean +/−SEM, standard error of the mean) of the expression levels (UMIs, unique molecular identifiers, normalized for the library size) of Rnf22, a marker of Oligo1. Differential expression analysis (DESeq 2) shows how Rnf22 expression is significantly higher in Oligo1 as compared to any other cell type (red line, p-values in Z-score scale, signed to indicate up/down-regulation). (c) Non-markers genes, such as Eif3c, do not show any significant (red line, Z-scores signed to indicate up/down-regulation) change of expression (UMIs, normalized for the library size) throughout the different cell types. (d) Heatmap representing oligodendrocytes markers: these 185 genes are expressed in all the 6 oligodendrocytes subtypes whilst silenced in the other cell types (DESeq 2 corrected p-values). (e) Histogram (mean +/−SEM) of the expression levels (UMIs, normalized for the library size) of Sgk2, a marker of all oligodendrocytes.