Table 2.

Apparent kinetic parameters for recombinant PcpE.

Co-substrate	Substrate	K m (mM)	k cat (s−1)	k cat /K m (s−1mM-1)
2-CMA*	NADH	0.13 ± 0.04	143.7 ± 15.3	1105.4
	NADPH	0.67 ± 0.75	29.7 ± 17.7	44.3
MA	NADH	0.44 ± 0.57	1.2 ± 0.7	2.7
	NADPH	0.52 ± 0.37	0.7 ± 0.2	1.3
NADH	2-CMA*	0.09 ± 0.03	17.6 ± 1.8	195.6
	MA	0.09 ± 0.04	1.2 ± 0.3	13.3

*No purification.